Collection of specimens for microbiology

Getting samples for microbiological tests are extremely important in obtaining specimens to achieve the right diagnosis by correct lab result An improperly taken sample may indicate bacteriological contamination and remember microbiological sample differ in the way of collection from other specimens  :

  1. Contamination from normal commensal is a misleading , avoid contaminants
  2. Good result need good amount of specimens (even any amount can be cultured but the probability of having true positive increase to the size of the sample)
    Getting samples for microbiological tests

    Getting samples for microbiological tests

Let us know the proper collection of specimens for microbiology


Microbiological specimens are same like any other lab specimens need to be well labelled even before being sent to the laboratory, patient’s full name, date of birth, medical Record Number source of specimen, collection date and time and diagnosis, antibiotics therapy. All of those data are so important for proper processing and to avoid mixing specimens


Remember … all body fluids are considered infectious apply standard precautions while dealing with body fluids you have to handle all specimens with care


Timely transport to the lab to start the process:

Never ever use needle and syringe to transport specimens it always can pose a risk of needle stick injury with all hazardous consequences and syringe itself can expel the content during transport …use proper specimen container to transport from the patient zone to the laboratory

Amniotic Fluid sampling for Microbiological Testing

In limited situations we need to culture the amniotic fluid either via amniocentesis or collection during the cesarean section sterile catheter and sterile container to collect the amniotic fluid, it is not acceptable to aspirate vaginal membrane as it can be contaminated with of vaginal contamination, specimens has to be transported in anaerobic transport system and to keep in the regular room temperature


The following articles will discuss in details the procedure to collect specimens and how to handle and transport as part of infection prevention and control awareness



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